goat anti mouse immunoglobulin g1 igg1 apc  (SouthernBiotech)

Journal: Cell reports

Article Title: Alpha synuclein, the culprit in Parkinson disease, is required for normal immune function

doi: 10.1016/j.celrep.2021.110090

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse APC/Cy7 Hamster IgG1 (clone G235-2356) , BD PharMingen , Cat# 561206.

Techniques: Recombinant, Stripping Membranes, SYBR Green Assay, Multiplex Assay, Software, Flow Cytometry, Light Microscopy

Journal: Developmental and comparative immunology

Article Title: CD9 expression in porcine blood CD4 + T cells delineates two subsets with phenotypic characteristics of central and effector memory cells.

doi: 10.1016/j.dci.2022.104431

Figure Lengend Snippet: Fig. 1. MAb 4H5CR4 recognizes porcine CD9. (A) Reactivity of mAb 4H5CR4 with PBMC subsets: PBMCs were stained with mAbs to CD3, CD4, CD8α, CD8β, γδTCR, CD16, CD21, CD172a, IgM or Siglec-10, and APC-conjugated-goat anti-mouse Ig (y-axis), followed by biotin-conjugated mAb 4H5CR4 and PE-streptavidin (x-axis). Quadrants were set by background staining with irrelevant isotype-matched mAbs. Numbers indicate the percentage of cells within the respective quadrants. Results are representative of at least three independent experiments with cells from 8 to 12-month-old pigs. (B) Molecular characterization: Lysates from alveolar macro phages were resolved by 12% SDS-PAGE under reducing conditions, transferred to nitrocellulose membranes, and probed with mAb 4H5CR4 (1) or an irrelevant isotype-matched mAb (2). Numbers in the right indicate position and size of MW markers. Results are representative of two independent experiments. (C) CHO cells transiently transfected with pCD9-GFP construct were stained with mAb 4H5CR4, or an IgG1 control antibody followed by APC-goat F(ab’)2 anti-mouse Igs and analyzed by flow cytometry. A control staining of non-transfected CHO cells is also shown.

Article Snippet: After a washing step with PBS, cells (106/well) were incubated with mAbs to different surface antigens (2E3, CD4, CD8α, 4H5CR4) for 30 min at 4 ◦C, followed by FITC-conjugated goat anti-mouse IgM, APC-conjugated goat anti-mouse IgG1, PE-Cy7-conjugated goat anti-mouse IgG2a (all from Southern Biotech) and BV421-conjugated goat anti-mouse IgG2b (Jackson B. Álvarez et al. Developmental and Comparative Immunology 133 (2022) 104431 ImmunoResearch Laboratories, Pennsylvania, USA).

Techniques: Staining, SDS Page, Transfection, Construct, Control, Flow Cytometry

Journal: Developmental and comparative immunology

Article Title: CD9 expression in porcine blood CD4 + T cells delineates two subsets with phenotypic characteristics of central and effector memory cells.

doi: 10.1016/j.dci.2022.104431

Figure Lengend Snippet: Fig. 3. CCR7 expression in CD4+ T cell subsets. (A) PBMC were stained with a combination of mAbs to CD4, 2E3, CD8α, and CD9, and recombinant pCCL19-Fc followed by fluorochrome-conjugated secondary goat antibodies specific for mouse Ig isotypes and biotin-conjugated goat anti-human IgG, and then BV421- streptavidin. CD4+ T cells were gated into 2E3+ and 2E3− cells and further subdivided according to CD8α vs CD9 expression. Filled histograms represent the binding of pCCL19-Fc to the gated subsets. Open histograms correspond to the staining with a control human IgG. (B) Median fluorescence intensity of CCR7 expression for each subset. Data of six animals are shown by individual symbols; symbols of same color correspond to the same animal. The mean is indicated by the black bar. Significant differences among groups are denoted by red bars on the top of the graph, p < 0.001.

Article Snippet: After a washing step with PBS, cells (106/well) were incubated with mAbs to different surface antigens (2E3, CD4, CD8α, 4H5CR4) for 30 min at 4 ◦C, followed by FITC-conjugated goat anti-mouse IgM, APC-conjugated goat anti-mouse IgG1, PE-Cy7-conjugated goat anti-mouse IgG2a (all from Southern Biotech) and BV421-conjugated goat anti-mouse IgG2b (Jackson B. Álvarez et al. Developmental and Comparative Immunology 133 (2022) 104431 ImmunoResearch Laboratories, Pennsylvania, USA).

Techniques: Expressing, Staining, Recombinant, Binding Assay, Control, Fluorescence